Variations in food texture and flavour can cause repeat customers to stop trusting the product or to make complaints about it. To prevent these problems, food manufacturing always needs to provide consumers with stable quality in texture and flavour. Quality that adds value to food can be evaluated by observing and analysing its appearance (for example, bubbles and ingredient crystals).
This section explains the importance of magnified observation and analysis of food. This section also introduces the latest examples of observation and analysis using our 4K digital microscope, such as observation and automatic analysis of gas cells in bread and chocolate bloom observation, which are difficult. These examples show how quantitative evaluation is now possible with simple operations.

Observation and Analysis of Bubbles, Chocolate Bloom, and Other Appearances Affecting Food Quality

Food Quality Evaluation by Appearance Observation and Analysis

It is extremely difficult for inspectors to use their senses to frequently evaluate textures and flavours of mass-produced foods. Quality preservation through quantitative evaluation is difficult at most manufacturing sites and can even be said to be impractical because it is difficult to secure inspectors having sensory testing skills and inspection results can vary from inspector to inspector and from day to day.
Therefore, it is effective to quantitatively evaluate food conditions, such as texture, flavour, and quality, by closely observing and analysing the food appearance using microscopes. The following are examples of food quality evaluation based on microscopic appearance, shapes, measured values, and other related factors.

Bread and cakes

Melting and smooth textures in the mouth can be compared and evaluated by observing and analysing the conditions of gas cells on cross sections of bread, bread doughs, cakes, and similar foods.

Chocolates

The appearance of solid chocolates is observed while they cool. In the manufacturing process, the appearance is checked to find whitish coating (or bloom*), which forms when certain ingredients melting in the chocolate cool and solidify on the surface.
Chocolate with lower surface roughness has better mouthfeel and melting texture. Because the surface roughness is affected by temperature after shipment, the surface roughness is controlled for the target season in the case of some seasonal products.

TipsWhat is chocolate bloom?
Chocolate bloom is a coating on chocolate. Some ingredients melt at certain temperatures and rise to the chocolate surface. Bloom forms, like flowers bloom, when these ingredients solidify and crystallise on the cooled surface. There are two types of chocolate bloom—fat bloom and sugar bloom. Fat bloom forms when fat melting in cocoa butter rises to the surface and solidifies. Sugar bloom forms when melting sugar rises to the surface and solidifies.
Note that too much bloom gives chocolate an overall whitish colour, which impairs not only its appearance but also its flavour.

Noodles including thick noodles, stir-fried noodles, and pasta

Smooth mouthfeel and the feeling when food is swallowed can be checked and evaluated by observing surface irregularities on noodles using a microscope.
The core conditions (water displacement) can also be checked by observing cross sections of noodles, which is effective in determining the appropriate boiling time. Additionally, observation and analysis of air bubbles in noodles can be used to determine freeze-drying conditions as well as to check the texture and boiling time.

Frozen foods including meat and fried foods

Frozen foods, such as Hamburg steaks, croquettes, and chicken cutlets, can be evaluated by checking the binding conditions of meat and between ingredients and coatings, the change over time caused by freezing and defrosting, ice crystals, and other related items using a microscope.
The crispy texture of fried food coatings can be evaluated by quantifying three-dimensional surface irregularities.

Foods (meat dishes) ready to eat at room temperature

Meat tenderness can be evaluated by observing simmered or roasted meat fibres. Additionally, textures can be evaluated by checking differences in the tissue shape and surface roughness caused by differences in factors such as content ratios of ingredients.

Ham and sausages

Changes in texture may be evaluated by observing changes in meat tissues before and after freezing and by quantifying analysed ratios of ingredients.

Tofu, soymilk, and similar foods

The processing quality can be evaluated by observing and analysing air bubbles (porosity) in cross sections. Smoothness can be evaluated by analysing proteins.

Sake and other alcoholic beverages

Mouthfeel and the feeling when beer is swallowed can be evaluated by measuring and quantifying the shape of gas bubbles in beer.
Some sake breweries visualise and quantify the core of sake rice to determine the quality of rice suitable for brewing daiginjo, sake made with rice of which at least 50% of the outer layers are polished away, and the precise polishing amount.

As introduced above, the quality of food, such as its texture and flavour, can be evaluated by observing and analysing its appearance.
Read on for introductions to examples of the improvement of sophistication and efficiency of food observation and analysis using our 4K digital microscope.

Observation and Analysis Examples for Evaluation of Food Textures and Quality Using Our 4K Digital Microscope

Processed foods come in a wide range because they use various ingredients having different light reflectance and absorptance and have three dimensional and complex shapes. Therefore, it is difficult to determine observation conditions and to adjust the focus, so these operations take a lot of time and effort. Visual analysis also has the problem that human errors in values hinder quantitative evaluation.

KEYENCE has developed a microscope that solves various problems arising from magnified observation and analysis, improving the sophistication and efficiency of these operations. The product in question is the VHX Series 4K digital microscope.
Examples of food observation and analysis using gas cells in bread dough and chocolate bloom are introduced below.

Observation of gas cells in bread dough

The VHX Series 4K digital microscope has both large depth of field and high resolution. With its observation system, the 4K CMOS image sensor and various functions can be used with simple operations, speeding up advanced observation.

The large depth of field of the VHX Series enables observation using clear 4K images that are fully focused on three dimensional targets like gas cells in bread dough.

The Multi-lighting function automatically obtains data of multiple images captured under omnidirectional lighting at the press of a button, enabling prompt observation simply by selecting the image suitable for the purpose. This significantly reduces time and effort required for condition determination.
Additionally, when an operator simply selects a past image, the conditions used for capturing that image are reproduced, allowing for comparison and evaluation using data acquired in observation under the same conditions even when a different sample of the same type of target is observed on a different day by a different operator.

Observation of gas cells in bread dough using the VHX Series 4K digital microscope
Multi-lighting (20x)
Multi-lighting (20x)

Automatic analysis of gas cells in bread dough

The VHX Series 4K digital microscope can seamlessly switch operation from observation using a captured high resolution 4K image to highly accurate automatic analysis when the operator simply selects an area.
Binarisation can be performed at arbitrary thresholds using the automatic area measurement/count function. This function automatically measures and calculates detailed values—such as the average area, standard deviation, maximum value, minimum value, area ratio, and other items of gas cells in a selected area as well as the accurate count, area, perimeter, maximum diameter, and minimum diameter of gas cells—and then displays these values in a list. This function quantifies the analysis results, eliminating variations in evaluation.

Additionally, Excel can be installed directly on the VHX Series, eliminating the need for a PC. With Excel installed, templates can be created in advance to output images and necessary values in the desired layout, allowing for automatic report creation.

Equipped with these various functions, the VHX Series can support the series of operations of lighting condition determination, focus adjustment, analysis, and report creation easily and quickly with a single unit.

Automatic analysis of gas cells in bread dough using the VHX Series 4K digital microscope
Multi-lighting illumination (20x) + Automatic area measurement/count
Multi-lighting illumination (20x) + Automatic area measurement/count

Observation of chocolate bloom

The VHX Series 4K digital microscope can capture clear 4K images of chocolate bloom for observation even at high magnifications.
Observation can be difficult when the food surface has low contrast between the normal part (the background) and the chocolate bloom (the observation target). The VHX Series can observe these targets with high contrast thanks to the High Dynamic Range (HDR) function, which captures multiple images at varying shutter speeds to obtain an image with high colour gradation.
It is also difficult to observe the entire bloom on a three-dimensional chocolate at high magnifications because only a part of the chocolate bloom can be brought into focus. The VHX Series is equipped with the depth composition function, which composes multiple images having different focus positions. This function can generate an image fully focused on the entire field of view even when the target has large height differences, enabling clear observation of the entire target.
With a wide range of functions for image capturing and processing, even chocolate bloom can be observed easily and quickly at high magnifications using clear 4K images.

Observation of chocolate bloom using the VHX Series 4K digital microscope
Ring illumination (500x)
Ring illumination (500x)

A 4K Digital Microscope That Improves the Efficiency of Food Texture and Flavour Evaluation Based on Appearance

Many foods are difficult to observe and analyse, requiring much time and effort in proportion to this difficulty.
The VHX Series 4K digital microscope provides high performance and functions that are accessible with simple operations, enabling observation using clear images and accurate automatic analysis quickly with a single unit.
Its automation technologies can be used for the series of operations of observation condition determination, focus adjustment, analysis, and report creation, significantly reducing the required man-hours.
Equipped with many other functions, the VHX Series can be used for a wide range of applications in the food industry such as observation and analysis of emulsion, crystals, and microorganism.

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